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Literature summary for 3.4.21.B10 extracted from

  • Blaber, S.I.; Yoon, H.; Scarisbrick, I.A.; Juliano, M.A.; Blaber, M.
    The autolytic regulation of human kallikrein-related peptidase 6 (2007), Biochemistry, 46, 5209-5217.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.B10

Activating Compound

Activating Compound Comment Organism Structure
additional information self-regulating activity of KLK6 is negative feedback inhibition via autolytic inactivation. Activation of pro-KLK6 may be achieved through the action of a separate activating protease (possibly plasmin or KLK5) Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant KLK6 expressed from an insect cell/baculovirus host. Pro-KLK6 expressed as a fusion construct with a C-terminal Strep-tag and 8x His-tag, respectively in HEK-293 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information self-regulating activity of KLK6 is negative feedback inhibition via autolytic inactivation Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25866
-
 x * 25866, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by sequential affinity chromatography utilizing Ni-NTA Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
o-aminobenzoic acid-EEQNKLVH-[2,4-dinitrophenyl]ethylenediamine + H2O low rates of hydrolysis of the pro-peptide substrate by KLK6 Homo sapiens ?
-
 ?
o-aminobenzoic acid-NLRQRESS-[2,4-dinitrophenyl]ethylenediamine + H2O autolysis loop substrate is hydrolyzed with 2-3 order of magnitude greater efficiency than the pro-peptide substrate Homo sapiens ?
-
 ?
pro-KLK6 + H2O KLK6 hydrolyzes its pro-sequence and internal autolysis site. Ability of KLK6 to activate pro-KLK6 is essentially negligible when compared to the rate of the internal autolytic inactivation or to the ability of other proteases to activate pro-KLK6 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 25866, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
kallikrein-related peptidase 6
-
 Homo sapiens
KLK6
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 8 at pH 6.0 the rate of hydrolysis of the autolysis loop substrate decreases 2fold, whereas the rate of hydrolysis of the pro-peptide substrate by KLK6 increases approximately 5fold, in comparison to pH 8.0. Autolysis loop substrate is still hydrolyzed with 2-3 order of magnitude greater efficiency in comparison to the pro-peptide substrate in this pH-range Homo sapiens